ABCB1 / MDR1 / P Glycoprotein Antibody (clone C219)

LifeSpan BioSciences

ABCB1 / MDR1 / P Glycoprotein Antibody (clone C219)

14

Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein; not species specific. Species Crossreactivity: human, mouse, rat and hamster. Crossreactivity Proteins

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Flow Cytometry: Cell permeabilization required. Western Blot: 1:25-1:50. Immunohistochemistry (Paraffin and Frozen): For paraffin sections, dilute 1:12.5 for biotin based detection. The antibody should be incubated for 60 minutes at RT. Immunocytochemistry: Cytospin preparations. Positive control: Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.. Flo, ICC, IHC, IHC-Fr, IHC-P, IP, WB (1:25 - 1:50) Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Flow Cytometry: Cell permeabilization required. Western Blot: 1:25-1:50. Immunohistochemistry (Paraffin and Frozen): For paraffin sections, dilute 1:12.5 for biotin based detection. The antibody should be incubated for 60 minutes at RT. Immunocytochemistry: Cytospin preparations. Positive control: Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.

Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

C219

Unconjugated

ABCB1

ATP binding cassette subfamily B member 1

ABC20; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-dependent translocase ABCB1; CD243; CLCS; colchicin sensitivity; doxorubicin resistance; GP170; MDR1; multidrug resistance protein 1; p-170; P-glycoprotein 1; P-GP; PGY1; phospholipid transporter ABCB1

Mouse

IgG1

Hamster, Human, Mouse, Rat

-20°C,2°C to 8°C

12352203