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P Glycoprotein antibody

GTX108354
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetABCB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    P Glycoprotein antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.58 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5243
  • Target name
    ABCB1
  • Target description
    ATP binding cassette subfamily B member 1
  • Target synonyms
    ABC20, CD243, CLCS, ENPAT, GP170, MDR1, P-GP, PGY1, p-170, ATP-dependent translocase ABCB1, ATP-binding cassette, sub-family B (MDR/TAP), member 1, P-glycoprotein 1, colchicin sensitivity, doxorubicin resistance, multidrug resistance protein 1, phospholipid transporter ABCB1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08183
  • Protein Name
    ATP-dependent translocase ABCB1
  • Scientific Description
    The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Onuma S, Manabe A, Yoshino Y, et al. Upregulation of Chemoresistance by Mg(2+) Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells. Cells. 2021,10(5). doi: 10.3390/cells10051179
    Read this paper
  • Maruhashi R, Eguchi H, Akizuki R, et al. Chrysin enhances anticancer drug-induced toxicity mediated by the reduction of claudin-1 and 11 expression in a spheroid culture model of lung squamous cell carcinoma cells. Sci Rep. 2019,9(1):13753. doi: 10.1038/s41598-019-50276-z
    Read this paper
  • Yan A, Joachims ML, Thompson LF, et al. CD73 Promotes Glioblastoma Pathogenesis and Enhances Its Chemoresistance via A(2B) Adenosine Receptor Signaling. J Neurosci. 2019,39(22):4387-4402. doi: 10.1523/JNEUROSCI.1118-18.2019
    Read this paper
  • Eguchi H, Akizuki R, Maruhashi R, et al. Increase in resistance to anticancer drugs involves occludin in spheroid culture model of lung adenocarcinoma A549 cells. Sci Rep. 2018,8(1):15157. doi: 10.1038/s41598-018-33566-w
    Read this paper
  • Meng Q, He X, Xie G, et al. MEK inhibitor enhances sensitivity to chemotherapeutic drugs in multidrug resistant hepatocellular carcinoma cells. Oncol Lett. 2017,14(3):3089-3095. doi: 10.3892/ol.2017.6494
    Read this paper
  • Kim DG, Bynoe MS. A2A adenosine receptor modulates drug efflux transporter P-glycoprotein at the blood-brain barrier. J Clin Invest. 2016,126(5):1717-33. doi: 10.1172/JCI76207
    Read this paper

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Figure 1. Western blot analysis of P Glycoprotein using anti-P Glycoprotein antibody (A00049-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P Glycoprotein antigen affinity purified polyclonal antibody (Catalog # A00049-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P Glycoprotein at approximately 160KD. The expected band size for P Glycoprotein is at 141KD.
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Whole cell extract (30 microg) was separated by 5% SDS-PAGE, and the membrane was blotted with P Glycoprotein antibody [C219] (GTX23364) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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