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ABCB10 Antibody

CSB-PA871059XA01DOA
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityPlant
TargetPGP10
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Overview

  • Supplier
    Cusabio
  • Product Name
    ABCB10 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID837611
  • Target name
    PGP10
  • Target description
    P-glycoprotein 10
  • Target synonyms
    AT1G10680, ABCB10, ATP-binding cassette B10, F20B24.12, F20B24_12, P-glycoprotein 10, P-glycoprotein 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9SGY1
  • Protein Name
    ABC transporter B family member 10
  • Reactivity
    Plant
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ABCB10 using anti-ABCB10 antibody (PB9971). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, Lane 2: COLO320 whole cell lysates, Lane 3: 22RV1 whole cell lysates, Lane 4: PANC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB10 antigen affinity purified polyclonal antibody (Catalog # PB9971) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB10 at approximately 79 kDa, 65 kDa. The expected band size for ABCB10 is at 79 kDa.
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