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ProductsBack/ABCB11 / BSEP Antibody

ABCB11 / BSEP Antibody

LS-C749679
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetABCB11
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ABCB11 / BSEP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 146kDa, while the observed MW by Western blot was 150kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 146kDa, while the observed MW by Western blot was 150kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.8 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8647
  • Target name
    ABCB11
  • Target description
    ATP binding cassette subfamily B member 11
  • Target synonyms
    ABC16, BRIC2, BSEP, PFIC-2, PFIC2, PGY4, SPGP, bile salt export pump, ABC member 16, MDR/TAP subfamily, ATP-binding cassette sub-family B member 11, ATP-binding cassette, sub-family B (MDR/TAP), member 11, progressive familial intrahepatic cholestasis 2, sister p-glycoprotein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABCB11 using anti-ABCB11 antibody (PB9414). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB11 antigen affinity purified polyclonal antibody (Catalog # PB9414) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)?with Tanon 5200 system. A specific band was detected for ABCB11 at approximately 146 kDa. The expected band size for ABCB11 is at 146 kDa.
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