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Lane 1: MCF-7 cell lysates probed with ABCB4 Monoclonal Antibody, Unconjugated (bsm-51673M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates probed with ABCB4 Monoclonal Antibody, Unconjugated (bsm-51673M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates probed with ABCB4 Monoclonal Antibody, Unconjugated (bsm-51673M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

ABCB4 Monoclonal Antibody

BSM-51673M
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetABCB4
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Overview

  • Supplier
    Bioss
  • Product Name
    ABCB4 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(WB=1:500-4000，)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Y5T3
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID5244
  • Target name
    ABCB4
  • Target description
    ATP binding cassette subfamily B member 4
  • Target synonyms
    ABC21, GBD1, ICP3, MDR2, MDR2/3, MDR3, PFIC-3, PGY3, phosphatidylcholine translocator ABCB4, ATP-binding cassette sub-family B member 4, ATP-binding cassette, sub-family B (MDR/TAP), member 4, P-glycoprotein 3, P-glycoprotein-3/multiple drug resistance-3, multidrug resistance protein 3, multiple drug resistance 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP21439
  • Protein Name
    Phosphatidylcholine translocator ABCB4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of ABCB4 using anti-ABCB4 antibody (PB9275). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody (Catalog # PB9275) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 150 kDa. The expected band size for ABCB4 is at 142 kDa.
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