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ProductsBack/ABCC4 / MRP4 Antibody

ABCC4 / MRP4 Antibody

LS-C331977
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetABCC4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ABCC4 / MRP4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 88kDa/96kDa/144kDa/149kDa, while the observed MW by Western blot was 170kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 88kDa/96kDa/144kDa/149kDa, while the observed MW by Western blot was 170kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10257
  • Target name
    ABCC4
  • Target description
    ATP binding cassette subfamily C member 4 (PEL blood group)
  • Target synonyms
    MOAT-B, MOATB, MRP4, ATP-binding cassette sub-family C member 4, MRP/cMOAT-related ABC transporter, bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4), canalicular multispecific organic anion transporter (ABC superfamily), multi-specific organic anion transporter B, multidrug resistance-associated protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MRP4 using anti-MRP4 antibody (A01596). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP4 antigen affinity purified polyclonal antibody (Catalog # A01596) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP4 at approximately 200-250 kDa. The expected band size for MRP4 is at 150 kDa.
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