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Goat anti-ABCC4 / MRP4

EB06538
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetABCC4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ABCC4 / MRP4 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10257
  • Target name
    ABCC4
  • Target description
    ATP binding cassette subfamily C member 4 (PEL blood group)
  • Target synonyms
    MOAT-B, MOATB, MRP4, ATP-binding cassette sub-family C member 4, MRP/cMOAT-related ABC transporter, bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4), canalicular multispecific organic anion transporter (ABC superfamily), multi-specific organic anion transporter B, multidrug resistance-associated protein 4
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005836.2. GeneIDs all Nonhuman: 170924 (rat);. Purification: Antigen affinity purified. Names and symbols: ABCC4; MRP4; MOATB; MOAT-B; EST170205; ATP-binding cassette, sub-family C (CFTR/MRP), member 4; canalicular multispecific organic anion transporter (ABC superfamily); Multidrug Resistance Protein 4; RP11-74A12.1; ATP-binding cassette, sub-family C, member
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Weerachayaphorn J, Luo Y, Mennone A, et al. Deleterious effect of oltipraz on extrahepatic cholestasis in bile duct-ligated mice. J Hepatol. 2014,60(1):160-6. doi: 10.1016/j.jhep.2013.08.015
    Read this paper
  • Montani M, Hermanns T, Müntener M, et al. Multidrug resistance protein 4 (MRP4) expression in prostate cancer is associated with androgen signaling and decreases with tumor progression. Virchows Arch. 2013,462(4):437-43. doi: 10.1007/s00428-013-1390-8
    Read this paper
  • Gori I, Rodriguez Y, Pellegrini C, et al. Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4). Fertil Steril. 2013,99(7):1965-73.e2. doi: 10.1016/j.fertnstert.2013.01.146
    Read this paper
  • Weerachayaphorn J, Mennone A, Soroka CJ, et al. Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine. Am J Physiol Gastrointest Liver Physiol. 2012,302(9):G925-36. doi: 10.1152/ajpgi.00263.2011
    Read this paper
  • Xu S, Weerachayaphorn J, Cai SY, et al. Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression. Am J Physiol Gastrointest Liver Physiol. 2010,299(1):G126-35. doi: 10.1152/ajpgi.00522.2010
    Read this paper

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Figure 1. Western blot analysis of MRP4 using anti-MRP4 antibody (A01596). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP4 antigen affinity purified polyclonal antibody (Catalog # A01596) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP4 at approximately 200-250 kDa. The expected band size for MRP4 is at 150 kDa.
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