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Western Blot analysis of NIH-3T3, 293 cells using ABCG2 Polyclonal Antibody.
Western Blot analysis of NIH-3T3, 293 cells using ABCG2 Polyclonal Antibody.
Western Blot analysis of NIH-3T3, 293 cells using ABCG2 Polyclonal Antibody.

ABCG2 Antibody

CSB-PA006120
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetABCG2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ABCG2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9429
  • Target name
    ABCG2
  • Target description
    ATP binding cassette subfamily G member 2 (JR blood group)
  • Target synonyms
    ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, CDw388, EST157481, GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1, broad substrate specificity ATP-binding cassette transporter ABCG2, ABC transporter, ATP binding cassette subfamily G member 2 (Junior blood group), ATP-binding cassette transporter G2, ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group), ATPbinding cassette transporter ABCG2, breast cancer resistance protein, broad substrate specificity ATP-binding cassette transporter ABCG2 isoform 1 (Junior blood group), mitoxantrone resistance-associated protein, multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2, placenta specific MDR protein, placenta-specific ATP-binding cassette transporter, urate exporter
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNQ0
  • Protein Name
    ATP-binding cassette sub-family G member 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BCRP/ABCG2 using anti-BCRP/ABCG2 antibody (A00457-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCRP/ABCG2 antigen affinity purified polyclonal antibody (Catalog # A00457-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCRP/ABCG2 at approximately 65-80KD. The expected band size for BCRP/ABCG2 is at 72KD.
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