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ProductsBack/ATP Binding Cassette Transporter G2 (ABCG2) Polyclonal Antibody

ATP Binding Cassette Transporter G2 (ABCG2) Polyclonal Antibody

CAU26758
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetABCG2
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Overview

  • Supplier
    Biomatik
  • Product Name
    ATP Binding Cassette Transporter G2 (ABCG2) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9429
  • Target name
    ABCG2
  • Target description
    ATP binding cassette subfamily G member 2 (Junior blood group)
  • Target synonyms
    ABC transporter; ABC15; ABCP; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group); BCRP; BCRP1; BMDP; breast cancer resistance protein; broad substrate specificity ATP-binding cassette transporter ABCG2; CD338; CDw338; EST157481; GOUT1; mitoxantrone resistance-associated protein; MRX; multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2; MXR; MXR1; MXR-1; placenta specific MDR protein; placenta-specific ATP-binding cassette transporter; UAQTL1; urate exporter
  • Host
    Rabbit
  • Protein IDQ9UNQ0
  • Protein Name
    ATP-binding cassette sub-family G member 2
  • Scientific Description
    The ATP Binding Cassette Transporter G2 (ABCG2) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCRP/ABCG2 using anti-BCRP/ABCG2 antibody (A00457-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCRP/ABCG2 antigen affinity purified polyclonal antibody (Catalog # A00457-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCRP/ABCG2 at approximately 65-80KD. The expected band size for BCRP/ABCG2 is at 72KD.
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