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Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: ABCG5 antibody at 8.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73, 29 kDa Observed band size: 73 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: ABCG5 antibody at 8.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73, 29 kDa Observed band size: 73 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: ABCG5 antibody at 8.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73, 29 kDa Observed band size: 73 kDa

ABCG5 Antibody

CSB-PA863956LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetABCG5
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Overview

  • Supplier
    Cusabio
  • Product Name
    ABCG5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64240
  • Target name
    ABCG5
  • Target description
    ATP binding cassette subfamily G member 5
  • Target synonyms
    ATP-binding cassette sub-family G member 5; ATP-binding cassette, sub-family G (WHITE), member 5; sterolin 1; STSL; STSL2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H222
  • Protein Name
    ATP-binding cassette sub-family G member 5
  • Scientific Description
    Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ABCG5 using anti-ABCG5 antibody (PB9415). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug, Lane 2: A549 Whole Cell Lysate at 40ug, Lane 3: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody (Catalog # PB9415) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG5 at approximately 73 kDa. The expected band size for ABCG5 is at 73 kDa.
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