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Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA875651LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA875651LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA875651LA01HU at dilution of 1:100

ABCG8 Antibody

CSB-PA875651LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetABCG8
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Overview

  • Supplier
    Cusabio
  • Product Name
    ABCG8 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64241
  • Target name
    ABCG8
  • Target description
    ATP binding cassette subfamily G member 8
  • Target synonyms
    ATP-binding cassette sub-family G member 8; ATP-binding cassette, sub-family G (WHITE), member 8; GBD4; sterolin 2; STSL; STSL1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H221
  • Protein Name
    ATP-binding cassette sub-family G member 8
  • Scientific Description
    Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ABCG8 using anti-ABCG8 antibody (A01482-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG8 antigen affinity purified polyclonal antibody (Catalog # A01482-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG8 at approximately 76 kDa. The expected band size for ABCG8 is at 76 kDa.
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