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ABCG8 Polyclonal Antibody

BS-10149R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetABCG8
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Overview

  • Supplier
    Bioss
  • Product Name
    ABCG8 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), ELISA(ELISA=1:5000-10000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID64241
  • Target name
    ABCG8
  • Target description
    ATP binding cassette subfamily G member 8
  • Target synonyms
    GBD4, STSL, STSL1, ATP-binding cassette sub-family G member 8, ATP-binding cassette, sub-family G (WHITE), member 8, sterolin 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H221
  • Protein Name
    ATP-binding cassette sub-family G member 8
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Li R, Liu Y, Shi J, et al. Diosgenin regulates cholesterol metabolism in hypercholesterolemic rats by inhibiting NPC1L1 and enhancing ABCG5 and ABCG8. Biochim Biophys Acta Mol Cell Biol Lipids. 2019,1864(8):1124-1133. doi: 10.1016/j.bbalip.2019.04.010
    Read this paper
  • Huang J, Wang Y, Ying C, et al. Effects of mulberry leaf on experimental hyperlipidemia rats induced by high-fat diet. Exp Ther Med. 2018,16(2):547-556. doi: 10.3892/etm.2018.6254
    Read this paper
  • Gao X, Zeng Y, Liu S, et al. Acute stress show great influences on liver function and the expression of hepatic genes associated with lipid metabolism in rats. Lipids Health Dis. 2013,12:118. doi: 10.1186/1476-511X-12-118
    Read this paper

Datasheet

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Figure 1. Western blot analysis of ABCG8 using anti-ABCG8 antibody (A01482-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG8 antigen affinity purified polyclonal antibody (Catalog # A01482-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG8 at approximately 76 kDa. The expected band size for ABCG8 is at 76 kDa.
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