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ABCG8 Antibody (N-Terminus, Biotin)

LS-C142147
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetABCG8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ABCG8 Antibody (N-Terminus, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500) The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID64241
  • Target name
    ABCG8
  • Target description
    ATP binding cassette subfamily G member 8
  • Target synonyms
    ATP-binding cassette sub-family G member 8; ATP-binding cassette, sub-family G (WHITE), member 8; GBD4; sterolin 2; STSL; STSL1
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABCG8 using anti-ABCG8 antibody (A01482-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG8 antigen affinity purified polyclonal antibody (Catalog # A01482-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG8 at approximately 76 kDa. The expected band size for ABCG8 is at 76 kDa.
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