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IHC-P analysis of formalin fixed human kidney tissue section using GTX54932 ABHD4 antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
IHC-P analysis of formalin fixed human kidney tissue section using GTX54932 ABHD4 antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
IHC-P analysis of formalin fixed human kidney tissue section using GTX54932 ABHD4 antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

ABHD4 antibody

GTX54932
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetABHD4
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Overview

  • Supplier
    GeneTex
  • Product Name
    ABHD4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID63874
  • Target name
    ABHD4
  • Target description
    abhydrolase domain containing 4, N-acyl phospholipase B
  • Target synonyms
    ABH4, (Lyso)-N-acylphosphatidylethanolamine lipase, abhydrolase domain-containing protein 4, alpha/beta hydrolase domain-containing protein 4, alpha/beta-hydrolase 4, lyso-N-acylphosphatidylethanolamine lipase, protein ABHD4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TB40
  • Protein Name
    Protein ABHD4
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ABHD4 using anti-ABHD4 antibody (A14435-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABHD4 antigen affinity purified polyclonal antibody (Catalog # A14435-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABHD4 at approximately 39 kDa. The expected band size for ABHD4 is at 39 kDa.
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