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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.

Anti-ABHD4 Antibody

HPA000600
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetABHD4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ABHD4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID63874
  • Target name
    ABHD4
  • Target description
    abhydrolase domain containing 4, N-acyl phospholipase B
  • Target synonyms
    ABH4, (Lyso)-N-acylphosphatidylethanolamine lipase, abhydrolase domain-containing protein 4, alpha/beta hydrolase domain-containing protein 4, alpha/beta-hydrolase 4, lyso-N-acylphosphatidylethanolamine lipase, protein ABHD4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TB40
  • Protein Name
    Protein ABHD4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ABHD4 using anti-ABHD4 antibody (A14435-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABHD4 antigen affinity purified polyclonal antibody (Catalog # A14435-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABHD4 at approximately 39 kDa. The expected band size for ABHD4 is at 39 kDa.
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