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The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA196653(ABI1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA196653(ABI1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA196653(ABI1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

ABI1 Antibody

CSB-PA196653
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetABI1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ABI1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10006
  • Target name
    ABI1
  • Target description
    abl interactor 1
  • Target synonyms
    Abelson interactor 1; ABI-1; abl interactor 1; abl-binding protein 4; ABLBP4; Abl-interactor protein 1 long; E3B1; eps8 SH3 domain-binding protein; interactor protein AblBP4; nap1 binding protein; NAP1BP; spectrin SH3 domain-binding protein 1; SSH3BP; SSH3BP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IZP0
  • Protein Name
    Abl interactor 1
  • Scientific Description
    This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interactions with Abelson tyrosine kinases. The encoded protein plays a role in macropinocytosis as a component of the WAVE2 complex, and also forms a complex with EPS8 and SOS1 that mediates signal transduction from Ras to Rac. This gene may play a role in the progression of several malignancies including melanoma, colon cancer and breast cancer, and a t(10;11) chromosomal translocation involving this gene and the MLL gene has been associated with acute myeloid leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 14
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody (PB9416). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3BP1/ABI1 antigen affinity purified polyclonal antibody (Catalog # PB9416) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSH3BP1/ABI1 at approximately 65 kDa. The expected band size for SSH3BP1/ABI1 is at 55 kDa.
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