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WB analysis of various sample lysates using GTX55488 ACAD9 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX55488 ACAD9 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX55488 ACAD9 antibody. Dilution : 1:1000 Loading : 25microg per lane

ACAD9 antibody

GTX55488
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetACAD9
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Overview

  • Supplier
    GeneTex
  • Product Name
    ACAD9 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID28976
  • Target name
    ACAD9
  • Target description
    acyl-CoA dehydrogenase family member 9
  • Target synonyms
    acyl-Coenzyme A dehydrogenase family, member 9; complex I assembly factor ACAD9, mitochondrial; MC1DN20; NPD002; very-long-chain acyl-CoA dehydrogenase VLCAD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H845
  • Protein Name
    Acyl-CoA dehydrogenase family member 9, mitochondrial
  • Scientific Description
    This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACAD9 using anti-ACAD9 antibody (A05223-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAD9 antigen affinity purified polyclonal antibody (Catalog # A05223-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAD9 at approximately 65 kDa. The expected band size for ACAD9 is at 69 kDa.
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