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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ACADVL antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 71, 69, 73 kDa Observed band size: 71 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ACADVL antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 71, 69, 73 kDa Observed band size: 71 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ACADVL antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 71, 69, 73 kDa Observed band size: 71 kDa

ACADVL Antibody

CSB-PA001129LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACADVL
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACADVL Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID37
  • Target name
    ACADVL
  • Target description
    acyl-CoA dehydrogenase very long chain
  • Target synonyms
    ACAD6; acyl-Coenzyme A dehydrogenase, very long chain; LCACD; very long-chain specific acyl-CoA dehydrogenase, mitochondrial; VLCAD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49748
  • Protein Name
    Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
  • Scientific Description
    Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accommodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACADVL using anti-ACADVL antibody (PB10023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADVL antigen affinity purified polyclonal antibody (Catalog # PB10023) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACADVL at approximately 70 kDa. The expected band size for ACADVL is at 70 kDa.
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