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ProductsBack/ACADVL antibody [N1C1]

ACADVL antibody [N1C1]

GTX114232
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetACADVL
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Overview

  • Supplier
    GeneTex
  • Product Name
    ACADVL antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID37
  • Target name
    ACADVL
  • Target description
    acyl-CoA dehydrogenase very long chain
  • Target synonyms
    ACAD6, LCACD, VLCAD, very long-chain specific acyl-CoA dehydrogenase, mitochondrial, acyl-Coenzyme A dehydrogenase, very long chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49748
  • Protein Name
    Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
  • Scientific Description
    The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Tong D, Schiattarella GG, Jiang N, et al. NAD(+) Repletion Reverses Heart Failure With Preserved Ejection Fraction. Circ Res. 2021,128(11):1629-1641. doi: 10.1161/CIRCRESAHA.120.317046
    Read this paper
  • Raimo S, Zura-Miller G, Fezelinia H, et al. Mitochondrial morphology, bioenergetics and proteomic responses in fatty acid oxidation disorders. Redox Biol. 2021,41:101923. doi: 10.1016/j.redox.2021.101923
    Read this paper
  • Chu H, Wu C, Zhao Q, et al. Quantitative proteomics identifies FOLR1 to drive sorafenib resistance via activating autophagy in hepatocellular carcinoma cells. Carcinogenesis. 2021,42(5):753-761. doi: 10.1093/carcin/bgab019
    Read this paper
  • Tenopoulou M, Doulias PT, Nakamoto K, et al. Oral nitrite restores age-dependent phenotypes in eNOS-null mice. JCI Insight. 2018,3(16):pii: 122156. doi: 10.1172/jci.insight.122156.
    Read this paper
  • Stein CS, Jadiya P, Zhang X, et al. Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency. Cell Rep. 2018,23(13):3710-3720.e8. doi: 10.1016/j.celrep.2018.06.002
    Read this paper
  • Tenopoulou M, Chen J, Bastin J, et al. Strategies for correcting very long chain acyl-CoA dehydrogenase deficiency. J Biol Chem. 2015,290(16):10486-94. doi: 10.1074/jbc.M114.635102
    Read this paper
  • Doulias PT, Tenopoulou M, Greene JL, et al. Nitric oxide regulates mitochondrial fatty acid metabolism through reversible protein S-nitrosylation. Sci Signal. 2013,6(256):rs1. doi: 10.1126/scisignal.2003252
    Read this paper

Datasheet

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Figure 1. Western blot analysis of ACADVL using anti-ACADVL antibody (PB10023). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADVL antigen affinity purified polyclonal antibody (Catalog # PB10023) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACADVL at approximately 70 kDa. The expected band size for ACADVL is at 70 kDa.
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