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ProductsBack/ACAT2 Mouse Monoclonal Antibody [Clone ID: OTI1C5]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2. Positive lysates LY417006 (100ug) and LC417006 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2. Positive lysates LY417006 (100ug) and LC417006 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2. Positive lysates LY417006 (100ug) and LC417006 (20ug) can be purchased separately from OriGene.

ACAT2 Mouse Monoclonal Antibody [Clone ID: OTI1C5]

CF501216
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetACAT2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    ACAT2 Mouse Monoclonal Antibody [Clone ID: OTI1C5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1C5
  • Gene ID39
  • Target name
    ACAT2
  • Target description
    acetyl-CoA acetyltransferase 2
  • Target synonyms
    acetyl-CoA acetyltransferase, cytosolic, acetoacetyl Coenzyme A thiolase, acetyl-CoA transferase-like protein, cytosolic acetoacetyl-CoA thiolase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ9BWD1
  • Protein Name
    Acetyl-CoA acetyltransferase, cytosolic
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ACAT2 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-ACAT2 antibody (A03245-1). Overlay histogram showing HepG2 cells stained with A03245-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACAT2 Antibody (A03245-1, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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