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Western Blot analysis of HuvEc cells using ACE1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using ACE1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using ACE1 Polyclonal Antibody

ACE Antibody

CSB-PA007661
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetACE
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACE Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1636
  • Target name
    ACE
  • Target description
    angiotensin I converting enzyme
  • Target synonyms
    ACE1; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; angiotensin-converting enzyme; carboxycathepsin; CD143; CD143 antigen; DCP; DCP1; dipeptidyl carboxypeptidase 1; dipeptidyl carboxypeptidase I; kininase II; peptidase P
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12821
  • Protein Name
    Angiotensin-converting enzyme
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACE using anti-ACE antibody (A00251-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE antigen affinity purified polyclonal antibody (Catalog # A00251-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE at approximately 150-180 kDa. The expected band size for ACE is at 150 kDa.
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