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Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-ACE1 Polyclonal Antibody, Unconjugated (bs-0439R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-ACE1 Polyclonal Antibody, Unconjugated (bs-0439R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-ACE1 Polyclonal Antibody, Unconjugated (bs-0439R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

ACE Polyclonal Antibody

BS-0439R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetACE
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Overview

  • Supplier
    Bioss
  • Product Name
    ACE Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1636
  • Target name
    ACE
  • Target description
    angiotensin I converting enzyme
  • Target synonyms
    ACE1, CD143, DCP, DCP1, angiotensin-converting enzyme, CD143 antigen, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, carboxycathepsin, dipeptidyl carboxypeptidase 1, dipeptidyl carboxypeptidase I, kininase II, peptidase P
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12821
  • Protein Name
    Angiotensin-converting enzyme
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Ginsenoside Rg3 Attenuates Angiotensin II-Mediated Renal Injury in Rats and Mice by Upregulating Angiotensin-Converting Enzyme 2 in the Renal Tissue. Liu H et al., 2019, Evid Based Complement Alternat Med
    Read this paper
  • Tanshinone IIA contributes to the pathogenesis of endometriosis via renin angiotensin system by regulating the dorsal root ganglion axon sprouting. Chen ZZ et al., 2020 Jan 1, Life Sci
    Read this paper
  • Exposure to diisononyl phthalate induced an increase in blood pressure through activation of the ACE/ AT1R axis and inhibition of NO production. Deng T et al., 2019 Jul, Toxicol Lett
    Read this paper
  • Di-(2-ethylhexyl) phthalate induced an increase in blood pressure via activation of ACE and inhibition of the bradykinin-NO pathway. Deng T et al., 2019 Apr, Environ Pollut
    Read this paper
  • Comparing the effects of diethylhexyl phthalate and dibutyl phthalate exposure on hypertension in mice. Xie X et al., 2019 Jun 15, Ecotoxicol Environ Saf
    Read this paper
  • Ginsenoside Rg3 induces ginsenoside Rb1-comparable cardioprotective effects independent of reducing blood pressure in spontaneously hypertensive rats. Jiang Y et al., 2017 Nov, Exp Ther Med
    Read this paper
  • Simultaneous targeting of ATM and Mcl-1 increases cisplatin sensitivity of cisplatin-resistant non-small cell lung cancer. Zhang F et al., 2017 Aug 3, Cancer Biol Ther
    Read this paper
  • Pulmonary artery denervation improves pulmonary arterial hypertension induced right ventricular dysfunction by modulating the local renin-angiotensin-aldosterone system. Liu C et al., 2016 Oct 10, BMC Cardiovasc Disord
    Read this paper

Datasheet

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Figure 1. Western blot analysis of ACE using anti-ACE antibody (A00251-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE antigen affinity purified polyclonal antibody (Catalog # A00251-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE at approximately 150-180 kDa. The expected band size for ACE is at 150 kDa.
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