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Acetylcholinesterase (ACHE) Monoclonal Antibody

CAU33840
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityPorcine
TargetACHE
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Overview

  • Supplier
    Biomatik
  • Product Name
    Acetylcholinesterase (ACHE) Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID43
  • Target name
    ACHE
  • Target description
    acetylcholinesterase (Yt blood group)
  • Target synonyms
    ACEE, ARACHE, N-ACHE, YT, acetylcholinesterase, Yt blood group, acetylcholinesterase (Cartwright blood group), apoptosis-related acetylcholinesterase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP22303
  • Protein Name
    Acetylcholinesterase
  • Scientific Description
    The Acetylcholinesterase (ACHE) Monoclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Porcine
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACHE using anti-ACHE antibody (PB9417). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: PANC Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACHE antigen affinity purified polyclonal antibody (Catalog # PB9417) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACHE at approximately 68 kDa. The expected band size for ACHE is at 68 kDa.
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