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Goat anti-ACHE

EB06659
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetACHE
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ACHE Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm, plasma membrane and nucleus of U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID43
  • Target name
    ACHE
  • Target description
    acetylcholinesterase (Cartwright blood group)
  • Target synonyms
    ACEE; acetylcholinesterase; acetylcholinesterase (Yt blood group); apoptosis-related acetylcholinesterase; ARACHE; N-ACHE; YT; Yt blood group
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000656.1; NP_001269378.1. Purification: Antigen affinity purified. Names and symbols: ACHE; YT; acetylcholinesterase (YT blood group); ARACHE; N-ACHE; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase
  • Reactivity Supplier
    Human, Rat, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACHE using anti-ACHE antibody (PB9417). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: PANC Whole Cell Lysate at 40ug, Lane 5: COLO320 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACHE antigen affinity purified polyclonal antibody (Catalog # PB9417) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACHE at approximately 68 kDa. The expected band size for ACHE is at 68 kDa.
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