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Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate All lanes: ACO1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 99 kDa Observed band size: 99 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate All lanes: ACO1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 99 kDa Observed band size: 99 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate All lanes: ACO1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 99 kDa Observed band size: 99 kDa

ACO1 Antibody

CSB-PA001161LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID48
  • Target name
    ACO1
  • Target description
    aconitase 1
  • Target synonyms
    ACONS, HEL60, IREB1, IREBP, IREBP1, IRP1, cytoplasmic aconitate hydratase, aconitase 1, soluble, aconitate hydratase, cytoplasmic, citrate hydro-lyase, cytoplasmic aconitase, cytosplasmic aconitase, epididymis luminal protein 60, epididymis secretory sperm binding protein, ferritin repressor protein, iron regulatory protein 1, iron-responsive element-binding protein 1, soluble aconitase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21399
  • Protein Name
    Cytoplasmic aconitate hydratase
  • Scientific Description
    Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Aconitase 1/ACO1 using anti-Aconitase 1/ACO1 antibody (A02781-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U20S wholel cell lysates, Lane 4: monkey liver tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aconitase 1/ACO1 antigen affinity purified polyclonal antibody (Catalog # A02781-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aconitase 1/ACO1 at approximately 98KD. The expected band size for Aconitase 1/ACO1 is at 98KD.
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