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ProductsBack/Aconitase 1 Recombinant Antibody, AbBy Fluor-647 Conjugated

Aconitase 1 Recombinant Antibody, AbBy Fluor-647 Conjugated

BSM-62433R-BF647
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetACO1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Aconitase 1 Recombinant Antibody, AbBy Fluor-647 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID48
  • Target name
    ACO1
  • Target description
    aconitase 1
  • Target synonyms
    aconitase 1, soluble; aconitate hydratase, cytoplasmic; ACONS; citrate hydro-lyase; cytoplasmic aconitase; cytoplasmic aconitate hydratase; cytosplasmic aconitase; epididymis luminal protein 60; epididymis secretory sperm binding protein; ferritin repressor protein; HEL60; IREB1; IREBP; IREBP1; iron regulatory protein 1; iron-responsive element-binding protein 1; IRP1; soluble aconitase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21399
  • Protein Name
    Cytoplasmic aconitate hydratase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aconitase 1/ACO1 using anti-Aconitase 1/ACO1 antibody (A02781-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U20S wholel cell lysates, Lane 4: monkey liver tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aconitase 1/ACO1 antigen affinity purified polyclonal antibody (Catalog # A02781-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aconitase 1/ACO1 at approximately 98KD. The expected band size for Aconitase 1/ACO1 is at 98KD.
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