Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] (GTX109736) diluted at 1:10000.
![Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 μg of Aconitase 2 antibody [C1C3] (GTX109736). Western blot analysis was performed using Aconitase 2 antibody [C1C3] (GTX109736). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109736/GTX109736_40030_20150604_IP_w_23060500_141.webp "Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 μg of Aconitase 2 antibody [C1C3] (GTX109736). Western blot analysis was performed using Aconitase 2 antibody [C1C3] (GTX109736). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] (GTX109736) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109736/GTX109736_40030_20150410_IFA_w_23060500_645.webp "Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] (GTX109736) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Aconitase 2 antibody [C1C3] (GTX109736) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109736/GTX109736_40030_20141024_IHC_M_w_23060500_597.webp "Aconitase 2 antibody [C1C3] detects Aconitase 2 protein at mitochondria on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Aconitase 2 antibody [C1C3] (GTX109736) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] (GTX109736) diluted at 1:10000.
Aconitase 2 antibody [C1C3]
GTX109736
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACO2
Overview
- SupplierGeneTex
- Product NameAconitase 2 antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.82 mg/ml
- ConjugateUnconjugated
- Gene ID50
- Target nameACO2
- Target descriptionaconitase 2
- Target synonymsACONM, HEL-S-284, ICRD, OCA8, OPA9, aconitate hydratase, mitochondrial, aconitase 2, mitochondrial, citrate hydro-lyase, epididymis secretory sperm binding protein Li 284, mitochondrial aconitase
- HostRabbit
- IsotypeIgG
- Protein IDQ99798
- Protein NameAconitate hydratase, mitochondrial
- Scientific DescriptionThe protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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