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Acp1 Polyclonal Antibody

CAC10619
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetACP1
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Overview

  • Supplier
    Biomatik
  • Product Name
    ACP1 Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID52
  • Target name
    ACP1
  • Target description
    acid phosphatase 1
  • Target synonyms
    HAAP, LMW-PTP, LMWPTP, low molecular weight phosphotyrosine protein phosphatase, LMW-PTPase, acid phosphatase 1, soluble, acid phosphatase of erythrocyte, adipocyte acid phosphatase, cytoplasmic phosphotyrosyl protein phosphatase, low molecular weight cytosolic acid phosphatase, protein tyrosine phosphatase, red cell acid phosphatase 1, testicular secretory protein Li 37
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24666
  • Protein Name
    Low molecular weight phosphotyrosine protein phosphatase
  • Scientific Description
    The Acp1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Acid phosphatase/ACP1 using anti-Acid phosphatase/ACP1 antibody (A02167-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hele whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse 4T1 whole cell lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Acid phosphatase/ACP1 antigen affinity purified polyclonal antibody (Catalog # A02167-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Acid phosphatase/ACP1 at approximately 17 kDa. The expected band size for Acid phosphatase/ACP1 is at 17 kDa.
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