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Paraformaldehyde-fixed, paraffin embedded human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Prostatic Acid Phosphatase Antibody (bs-6293R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Prostatic Acid Phosphatase Antibody (bs-6293R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Prostatic Acid Phosphatase Antibody (bs-6293R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Prostatic Acid Phosphatase Antibody

BS-6293R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetACP1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Prostatic Acid Phosphatase Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID52
  • Target name
    ACP1
  • Target description
    acid phosphatase 1
  • Target synonyms
    acid phosphatase 1, soluble; acid phosphatase of erythrocyte; adipocyte acid phosphatase; cytoplasmic phosphotyrosyl protein phosphatase; HAAP; LMWPTP; LMW-PTP; LMW-PTPase; low molecular weight cytosolic acid phosphatase; low molecular weight phosphotyrosine protein phosphatase; protein tyrosine phosphatase; red cell acid phosphatase 1; testicular secretory protein Li 37
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24666
  • Protein Name
    Low molecular weight phosphotyrosine protein phosphatase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Acid phosphatase/ACP1 using anti-Acid phosphatase/ACP1 antibody (A02167-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hele whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse 4T1 whole cell lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Acid phosphatase/ACP1 antigen affinity purified polyclonal antibody (Catalog # A02167-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Acid phosphatase/ACP1 at approximately 17 kDa. The expected band size for Acid phosphatase/ACP1 is at 17 kDa.
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