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ProductsBack/ACPL2 antibody, Internal
WB analysis of THP-1 cells using GTX46615 ACPL2 antibody at 0.2-1microg/ml.
WB analysis of THP-1 cells using GTX46615 ACPL2 antibody at 0.2-1microg/ml.
WB analysis of THP-1 cells using GTX46615 ACPL2 antibody at 0.2-1microg/ml.

ACPL2 antibody, Internal

GTX46615
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPXYLP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ACPL2 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID92370
  • Target name
    PXYLP1
  • Target description
    2-phosphoxylose phosphatase 1
  • Target synonyms
    ACPL2, HEL124, XYLP, 2-phosphoxylose phosphatase 1, acid phosphatase-like 2, acid phosphatase-like protein 2, epididymis luminal protein 124, xylosyl phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TE99
  • Protein Name
    2-phosphoxylose phosphatase 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACPL2/PXYLP1 using anti-ACPL2/PXYLP1 antibody (A13883-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACPL2/PXYLP1 antigen affinity purified polyclonal antibody (Catalog # A13883-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACPL2/PXYLP1 at approximately 70 kDa. The expected band size for ACPL2/PXYLP1 is at 55 kDa.
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