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Immunohistochemical staining of human epididymis shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human epididymis shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human epididymis shows moderate cytoplasmic positivity in glandular cells.

Anti-PXYLP1 Antibody

HPA017243
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPXYLP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PXYLP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92370
  • Target name
    PXYLP1
  • Target description
    2-phosphoxylose phosphatase 1
  • Target synonyms
    ACPL2, HEL124, XYLP, 2-phosphoxylose phosphatase 1, acid phosphatase-like 2, acid phosphatase-like protein 2, epididymis luminal protein 124, xylosyl phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TE99
  • Protein Name
    2-phosphoxylose phosphatase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ACPL2/PXYLP1 using anti-ACPL2/PXYLP1 antibody (A13883-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACPL2/PXYLP1 antigen affinity purified polyclonal antibody (Catalog # A13883-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACPL2/PXYLP1 at approximately 70 kDa. The expected band size for ACPL2/PXYLP1 is at 55 kDa.
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