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ProductsBack/ACPP / PAP Antibody (clone 019, Preservative Free)

ACPP / PAP Antibody (clone 019, Preservative Free)

LS-C819384
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetACP3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACPP / PAP Antibody (clone 019, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA(Cap): This antibody will detect Mouse Prostatic Acid Phosphatase / ACPP in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Applications
    ELISA
  • Applications Supplier
    ELISA ELISA(Cap): This antibody will detect Mouse Prostatic Acid Phosphatase / ACPP in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    019
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55
  • Target name
    ACP3
  • Target description
    acid phosphatase 3
  • Target synonyms
    5'-NT, ACP-3, ACPP, TM-PAP, prostatic acid phosphatase, TMPase, acid phosphatase, prostate, ecto-5'-nucleotidase, prostatic acid phosphotase, protein tyrosine phosphatase ACP3, thiamine monophosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAP/ACP3 antigen affinity purified polyclonal antibody (Catalog # A02082-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAP/ACP3 at approximately 45-50 kDa. The expected band size for PAP/ACP3 is at 45 kDa.
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