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ProductsBack/ACSL3 + ACSL4 antibody [HL1941]
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL3 + ACSL4 antibody [HL1941] (GTX637778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL3 + ACSL4 antibody [HL1941] (GTX637778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL3 + ACSL4 antibody [HL1941] (GTX637778) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ACSL3 + ACSL4 antibody [HL1941]

GTX637778
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetACSL3
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Overview

  • Supplier
    GeneTex
  • Product Name
    ACSL3 + ACSL4 antibody [HL1941]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1941
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2181
  • Target name
    ACSL3
  • Target description
    acyl-CoA synthetase long chain family member 3
  • Target synonyms
    ACS3, FACL3, LACS 3, LACS3, PRO2194, fatty acid CoA ligase Acsl3, arachidonate--CoA ligase, fatty-acid-Coenzyme A ligase, long-chain 3, lignoceroyl-CoA synthase, long-chain acyl-CoA synthetase 3, long-chain-fatty-acid--CoA ligase 3, medium-chain acyl-CoA ligase Acsl3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60488
  • Protein Name
    Long-chain-fatty-acid--CoA ligase 4
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACSL3 using anti-ACSL3 antibody (A05945). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSL3 antigen affinity purified polyclonal antibody (Catalog # A05945) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSL3 at approximately 80KD. The expected band size for ACSL3 is at 80KD.
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