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ACSL3 antibody

GTX112431
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetACSL3
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Overview

  • Supplier
    GeneTex
  • Product Name
    ACSL3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.81 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2181
  • Target name
    ACSL3
  • Target description
    acyl-CoA synthetase long chain family member 3
  • Target synonyms
    ACS3; arachidonate--CoA ligase; FACL3; fatty-acid-Coenzyme A ligase, long-chain 3; LACS 3; LACS3; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 3; long-chain-fatty-acid--CoA ligase 3; PRO2194
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95573
  • Protein Name
    Long-chain-fatty-acid--CoA ligase 3
  • Scientific Description
    The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Syntaxin 17 promotes lipid droplet formation by regulating the distribution of acyl-CoA synthetase 3. Kimura H et al., 2018 May, J Lipid Res
    Read more

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Figure 1. Western blot analysis of ACSL3 using anti-ACSL3 antibody (A05945). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSL3 antigen affinity purified polyclonal antibody (Catalog # A05945) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSL3 at approximately 80KD. The expected band size for ACSL3 is at 80KD.
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