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ACSM3 / SAH Antibody

LS-C812871
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetACSM3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACSM3 / SAH Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6296
  • Target name
    ACSM3
  • Target description
    acyl-CoA synthetase medium chain family member 3
  • Target synonyms
    acyl-coenzyme A synthetase ACSM3, mitochondrial; butyrate--CoA ligase 3; butyryl-coenzyme A synthetase 3; middle-chain acyl-CoA synthetase 3; propionate--CoA ligase; protein SA homolog; SA; SA (rat hypertension-associated) homolog; SA hypertension-associated homolog; SAH
  • Host
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAH/ACSM3 using anti-SAH/ACSM3 antibody (A08748-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAH/ACSM3 antigen affinity purified polyclonal antibody (Catalog # A08748-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAH/ACSM3 at approximately 66 kDa. The expected band size for SAH/ACSM3 is at 66 kDa.
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