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WB analysis of various sample lysates using GTX30020 ACSS2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX30020 ACSS2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX30020 ACSS2 antibody. Dilution : 1:1000 Loading : 25microg per lane

ACSS2 antibody

GTX30020
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetACSS2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ACSS2 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55902
  • Target name
    ACSS2
  • Target description
    acyl-CoA synthetase short chain family member 2
  • Target synonyms
    ACAS2; ACECS; AceCS1; acetate thiokinase; acetate-CoA ligase; acetyl-CoA synthetase 1; acetyl-Coenzyme A synthetase 2 (ADP forming); acetyl-coenzyme A synthetase, cytoplasmic; ACS; ACSA; acyl-activating enzyme; cytoplasmic acetyl-coenzyme A synthetase; dJ1161H23.1; propionate--CoA ligase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR19
  • Protein Name
    Acetyl-coenzyme A synthetase, cytoplasmic
  • Scientific Description
    This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Dual-tracer positron emission tomography/computed tomography as an imaging probe of de novo lipogenesis in preclinical models of hepatocellular carcinoma.
    Read more
  • Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy. Li X et al., 2017 Jun 1, Mol Cell
    Read more

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Figure 1. Western blot analysis of ACSS2 using anti-ACSS2 antibody (A02809-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSS2 antigen affinity purified polyclonal antibody (Catalog # A02809-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSS2 at approximately 79 kDa. The expected band size for ACSS2 is at 79 kDa.
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