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Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Anti-ACSS2 Antibody

HPA004141
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACSS2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACSS2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55902
  • Target name
    ACSS2
  • Target description
    acyl-CoA synthetase short chain family member 2
  • Target synonyms
    ACAS2, ACECS, ACS, ACSA, AceCS1, dJ1161H23.1, acetyl-coenzyme A synthetase, cytoplasmic, acetate thiokinase, acetate-CoA ligase, acetyl-CoA synthetase 1, acetyl-Coenzyme A synthetase 2 (ADP forming), acyl-activating enzyme, cytoplasmic acetyl-coenzyme A synthetase, propionate--CoA ligase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR19
  • Protein Name
    Acetyl-coenzyme A synthetase, cytoplasmic
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACSS2 using anti-ACSS2 antibody (A02809-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSS2 antigen affinity purified polyclonal antibody (Catalog # A02809-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSS2 at approximately 79 kDa. The expected band size for ACSS2 is at 79 kDa.
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