Actin antibody [BA3R]

GeneTex

Actin antibody [BA3R]

9

Recognizes native and denatured forms of β-Actin (~42kDa)

Recommended Starting Dilutions:WB (with ECL): Use at a dilution of 1:1000-10000 Immunostaining: Use at a dilution of 1:500 - 1:2000For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

Monoclonal

BA3R

1 mg/ml

Unconjugated

ACTB

actin beta

actin, cytoplasmic 1; beta cytoskeletal actin; BRWS1; I(2)-actin; PS1TP5-binding protein 1; PS1TP5BP1

Mouse

IgG2b

Actin is a ubiquitous globular protein that is one of the most highly-conserved proteins known. It is foundin two main states; G-actin is the globular monomeric form, whereas F-actin forms helical polymers. Both G-and F-actin are intrinsically flexible structures - a feature vital in actins role as a dynamic filamentnetwork. Actin has four major functions. Firstly, F-actin polymers form microfilaments - polar intracellulartracks for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actinis a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherensjunctions. This gives mechanical support to cells and attaches them to each other and the extracellularmatrix. In muscle cells, actin-rich thin filaments associate with myosin-rich thick filaments to formactomyosin myofibrils. Using energy from the hydrolysis of ATP, myofibrils undergo cyclic shortening throughactin-myosin head interactions, which represents the mechanics of muscle contraction. Finally, actin has arole in cell motility through polymerization and depolymerization of fibrils.

Chicken, Human, Mouse, Rabbit, Rat

-20°C or -80°C,2°C to 8°C

12352203