IHC-P analysis of human colon carcinoma tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000
![IHC-P analysis of human skeletal muscle tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX80809/GTX80809_1411_IHC-P_w_23061322_410.webp "IHC-P analysis of human skeletal muscle tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000")
![IHC-P analysis of human tonsil tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX80809/GTX80809_1412_IHC-P_w_23061322_520.webp "IHC-P analysis of human tonsil tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000")
![ICC/IF analysis of 3T3 cells using GTX80809 Actin antibody [mAbGEa]. Green : Primary antibody Blue : cell nucleus Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX80809/GTX80809_904_ICC-IF_w_23061322_169.webp "ICC/IF analysis of 3T3 cells using GTX80809 Actin antibody [mAbGEa]. Green : Primary antibody Blue : cell nucleus Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature")
![ICC/IF analysis of A375 Cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX80809/GTX80809_905_ICC-IF_w_23061322_233.webp "ICC/IF analysis of A375 Cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX80809/GTX80809_906_ICC-IF_w_23061322_285.webp "ICC/IF analysis of HeLa cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
IHC-P analysis of human colon carcinoma tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000
Actin antibody [mAbGEa]
GTX80809
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBacteria, Bovine, Drosophila, Human, Mouse, Plant, Porcine, Rat, Sheep, Xenopus, Yeast, Zebra Fish
TargetACTC1
Overview
- SupplierGeneTex
- Product NameActin antibody [mAbGEa]
- Delivery Days Customer9
- Antibody SpecificityThis antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.
- Application Supplier NoteWB: 1:1,000. ICC/IF: 1:10-1:100. IHC-P: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDmAbGEa
- ConjugateUnconjugated
- Gene ID70
- Target nameACTC1
- Target descriptionactin alpha cardiac muscle 1
- Target synonymsACTC; actin, alpha cardiac muscle 1; ASD5; CMD1R; CMH11; LVNC4
- HostMouse
- IsotypeIgG1
- Protein IDP68032
- Protein NameActin, alpha cardiac muscle 1
- Scientific DescriptionActins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]
- ReactivityBacteria, Bovine, Drosophila, Human, Mouse, Plant, Porcine, Rat, Sheep, Xenopus, Yeast, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis. Zhang T et al., 2020 Mar 19, CellRead more
- Hypoxia increases chemoresistance in human medulloblastoma DAOY cells via hypoxia-inducible factor 1alpha-mediated downregulation of the CYP2B6, CYP3A4 and CYP3A5 enzymes and inhibition of cell proliferation. Valencia-Cervantes J et al., 2019 Jan, Oncol RepRead more
- Curing of the [URE3] prion by Btn2p, a Batten disease-related protein. Kryndushkin DS et al., 2008 Oct 22, EMBO JRead more