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ProductsBack/Actin Related Protein 2 Antibody (YA2725, Rabbit)

Actin Related Protein 2 Antibody (YA2725, Rabbit)

HY-P82980
MedChem Express
TargetACTR2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Actin Related Protein 2 Antibody (YA2725, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10097
  • Target name
    ACTR2
  • Target description
    actin related protein 2
  • Target synonyms
    ARP2, actin-related protein 2, ARP2 actin related protein 2 homolog, actin-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Actin Related Protein 2 Antibody (YA2725) is a rabbit-derived non-conjugated IgG monoclonal antibody (Clone NO.: YA2725), targeting Actin Related Protein 2. It can be used as a loading control antibody.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Western blot All lanes: Actin-related protein 2 antibody at 3ug/ml Lane 1: Mouse spleen tissue Lane 2: Jurkat whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 46 kDa Observed band size: 45 kDa
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Figure 1. Western blot analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (A03898-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse HEPA1-6 wholel cell lysates, Lane 4: mouse SP2/0 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human PC-3 whole cell lysates, Lane 9: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arp2/ACTR2 antigen affinity purified polyclonal antibody (Catalog # A03898-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arp2/ACTR2 at approximately 45KD. The expected band size for Arp2/ACTR2 is at 45KD.
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