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ACTR3 Antibody (Preservative Free)

LS-C817750
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetACTR3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACTR3 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), ICC (1:300 - 1:10000), IF (1:300 - 1:10000), IP, WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10096
  • Target name
    ACTR3
  • Target description
    actin related protein 3
  • Target synonyms
    ARP3, actin-related protein 3, ARP3 actin related protein 3 homolog, actin-like protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Arp3/ACTR3 using anti-Arp3/ACTR3 antibody (A03423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole lysates, Lane 3: human U20S wholel cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat spleen tissue lysates, Lane 6: mose spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arp3/ACTR3 antigen affinity purified polyclonal antibody (Catalog # A03423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arp3/ACTR3 at approximately 47KD. The expected band size for Arp3/ACTR3 is at 47KD.
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