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ProductsBack/ACY1 / Aminoacylase 1 Antibody (clone OTI1B12)

ACY1 / Aminoacylase 1 Antibody (clone OTI1B12)

LS-C173144
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetACY1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACY1 / Aminoacylase 1 Antibody (clone OTI1B12)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IHC, IHC-P (1:150), WB (1:200 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1B12
  • Concentration
    0.28 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID95
  • Target name
    ACY1
  • Target description
    aminoacylase 1
  • Target synonyms
    ACY-1; ACY1D; acylase; aminoacylase-1; epididymis secretory protein Li 5; HEL-S-5; N-acyl-L-amino-acid amidohydrolase
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Rat Spleen lysates; Lane 2: Rat Small intestine lysates probed with Aminoacylase 1 Polyclonal Antibody, Unconjugated (bs-6019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of ACY-1/ACY1 using anti-ACY-1/ACY1 antibody (A05650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat brian tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACY-1/ACY1 antigen affinity purified polyclonal antibody (Catalog # A05650-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACY-1/ACY1 at approximately 40-43 kDa. The expected band size for ACY-1/ACY1 is at 46 kDa.
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