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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in renal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in renal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in renal tubules.

Anti-ACY1 Antibody

HPA036174
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACY1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACY1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID95
  • Target name
    ACY1
  • Target description
    aminoacylase 1
  • Target synonyms
    ACY-1, ACY1D, HEL-S-5, aminoacylase-1, N-acyl-L-amino-acid amidohydrolase, acylase, epididymis secretory protein Li 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03154
  • Protein Name
    Aminoacylase-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ACY-1/ACY1 using anti-ACY-1/ACY1 antibody (A05650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat brian tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACY-1/ACY1 antigen affinity purified polyclonal antibody (Catalog # A05650-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACY-1/ACY1 at approximately 40-43 kDa. The expected band size for ACY-1/ACY1 is at 46 kDa.
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