Bio-Connect

ADA antibody

70R-5745
Biosynth
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Biosynth
  • Product Name
    ADA antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
TargetADA
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ELISA
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
TargetADA
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetADA
  • SizePrice
Product group Antibodies
Assay Genie
TargetADA
  • SizePrice
Western blot All lanes: ADA antibody at 2microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa
Product group Antibodies
Cusabio
ADA AntibodyCSB-PA001268EA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetADA
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetADA
  • SizePrice
Figure 1. Western blot analysis of ADA using anti-ADA antibody (PB9914). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody (Catalog # PB9914) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for ADA at approximately 41 kDa. The expected band size for ADA is at 41 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetADA
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetADA
  • SizePrice