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Western blot All lanes: ADA antibody at 2microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa
Western blot All lanes: ADA antibody at 2microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa
Western blot All lanes: ADA antibody at 2microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa

ADA Antibody

CSB-PA001268EA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetADA
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADA Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID100
  • Target name
    ADA
  • Target description
    adenosine deaminase
  • Target synonyms
    ADA1; adenosine aminohydrolase; adenosine deaminase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00813
  • Protein Name
    Adenosine deaminase
  • Scientific Description
    Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Immunohistochemistry analysis in human duodenum and testis tissues using HPA001399 antibody. Corresponding ADA RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of ADA using anti-ADA antibody (PB9914). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody (Catalog # PB9914) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for ADA at approximately 41 kDa. The expected band size for ADA is at 41 kDa.
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