Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104940/GTX104940_40051_20200424_WB_M_brain_w_23060120_521.webp "Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Boiled and unboiled RT-4 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.(WCE: whole cell extract; ME: membrane extract)](https://www.genetex.com/upload/website/prouct_img/normal/GTX104940/GTX104940_45588_20250103_WB_Fraction_ub_25010819_971.webp "Boiled and unboiled RT-4 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.(WCE: whole cell extract; ME: membrane extract)")
![Untreated (–) and treated (+) unboiled A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104940/GTX104940_45588_20250103_WB_treatment_PNGaseF_25010819_898.webp "Untreated (–) and treated (+) unboiled A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ADAM10 antibody [N2C1], Internal
GTX104940
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityDrosophila, Human, Mouse
TargetADAM10
Overview
- SupplierGeneTex
- Product NameADAM10 antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.65 mg/ml
- ConjugateUnconjugated
- Gene ID102
- Target nameADAM10
- Target descriptionADAM metallopeptidase domain 10
- Target synonymsAD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz, disintegrin and metalloproteinase domain-containing protein 10, a disintegrin and metalloprotease domain 10, kuzbanian protein homolog, mammalian disintegrin-metalloprotease
- HostRabbit
- IsotypeIgG
- Protein IDO14672
- Protein NameDisintegrin and metalloproteinase domain-containing protein 10
- Scientific DescriptionMembers of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq]
- ReactivityDrosophila, Human, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhang Y, Hu R, Xi B, et al. Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells. Front Cell Dev Biol. 2022,10:829404. doi: 10.3389/fcell.2022.829404Read this paper
- Groot AJ, Habets R, Yahyanejad S, et al. Regulated proteolysis of NOTCH2 and NOTCH3 receptors by ADAM10 and presenilins. Mol Cell Biol. 2014,34(15):2822-32. doi: 10.1128/MCB.00206-14Read this paper
Datasheet
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