More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ADAM10 antibody [N2C1], Internal

ADAM10 antibody [N2C1], Internal

GTX104940
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetADAM10
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    ADAM10 antibody [N2C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.65 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID102
  • Target name
    ADAM10
  • Target description
    ADAM metallopeptidase domain 10
  • Target synonyms
    AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz, disintegrin and metalloproteinase domain-containing protein 10, a disintegrin and metalloprotease domain 10, kuzbanian protein homolog, mammalian disintegrin-metalloprotease
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14672
  • Protein Name
    Disintegrin and metalloproteinase domain-containing protein 10
  • Scientific Description
    Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhang Y, Hu R, Xi B, et al. Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells. Front Cell Dev Biol. 2022,10:829404. doi: 10.3389/fcell.2022.829404
    Read this paper
  • Groot AJ, Habets R, Yahyanejad S, et al. Regulated proteolysis of NOTCH2 and NOTCH3 receptors by ADAM10 and presenilins. Mol Cell Biol. 2014,34(15):2822-32. doi: 10.1128/MCB.00206-14
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

WB analysis of Jurkat cell lysate using GTX21997 ADAM10 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
Product group Antibodies
GeneTex
ADAM10 antibodyGTX21997
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetADAM10
  • SizePrice
Product group Antibodies
GeneTex
ADAM10 antibody [N1N2], N-termGTX111347
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetADAM10
  • SizePrice
WB analysis of various sample lysates using GTX64403 ADAM10 antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
ADAM10 antibodyGTX64403
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetADAM10
  • SizePrice
Immunofluorescence staining of Hela cells with CSB-PA001273LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
ADAM10 AntibodyCSB-PA001273LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetADAM10
  • SizePrice
Product group Antibodies
MedChem Express
ADAM10 Antibody (YA3292, Rabbit)HY-P83547
TargetADAM10
  • SizePrice
Product group Antibodies
GeneTex
ADAM10 antibody [6D31]GTX52528
ApplicationsWestern Blot
TargetADAM10
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ADAM10 Antibody144-10438
ApplicationsImmunoFluorescence, Western Blot
TargetADAM10
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-ADAM10Y058008
ApplicationsWestern Blot, ELISA
  • SizePrice
Anti-ADAM10 Antibody
Product group Antibodies
Antibodies.com
Anti-ADAM10 AntibodyA28523
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Figure 1. Western blot analysis of ADAM10 using anti-ADAM10 antibody (A00566-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM10 antigen affinity purified polyclonal antibody (Catalog # A00566-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM10 at approximately 72KD. The expected band size for ADAM10 is at 84KD.
Product group Antibodies
Boster Bio
Anti-ADAM10 Antibody Picoband(r)A00566-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetADAM10
  • SizePrice