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Immunohistochemistry analysis in human prostate and skeletal muscle tissues using AMAb91898 antibody. Corresponding ADAM10 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human prostate and skeletal muscle tissues using AMAb91898 antibody. Corresponding ADAM10 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human prostate and skeletal muscle tissues using AMAb91898 antibody. Corresponding ADAM10 RNA-seq data are presented for the same tissues.

Anti-ADAM10 Antibody

AMAB91898
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetADAM10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ADAM10 Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL13808
  • Conjugate
    Unconjugated
  • Gene ID102
  • Target name
    ADAM10
  • Target description
    ADAM metallopeptidase domain 10
  • Target synonyms
    AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz, disintegrin and metalloproteinase domain-containing protein 10, a disintegrin and metalloprotease domain 10, kuzbanian protein homolog, mammalian disintegrin-metalloprotease
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO14672
  • Protein Name
    Disintegrin and metalloproteinase domain-containing protein 10
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ADAM10 using anti-ADAM10 antibody (A00566-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM10 antigen affinity purified polyclonal antibody (Catalog # A00566-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM10 at approximately 72KD. The expected band size for ADAM10 is at 84KD.
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