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K562 lysates probed with ADAM2 Polyclonal Antibody, Unconjugated (bs-20273R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
K562 lysates probed with ADAM2 Polyclonal Antibody, Unconjugated (bs-20273R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
K562 lysates probed with ADAM2 Polyclonal Antibody, Unconjugated (bs-20273R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

ADAM2 Polyclonal Antibody

bs-20273R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetADAM2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ADAM2 Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2515
  • Target name
    ADAM2
  • Target description
    ADAM metallopeptidase domain 2
  • Target synonyms
    cancer/testis antigen 15; CRYN1; CRYN2; CT15; disintegrin and metalloproteinase domain-containing protein 2; fertilin subunit beta; FTNB; PH30; PH-30b; PH30-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99965
  • Protein Name
    Disintegrin and metalloproteinase domain-containing protein 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAM2 using anti-ADAM2 antibody (A08379-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat testis tissue lysate, lane 2: mouse testis tissue lysate, lane 3: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM2 antigen affinity purified polyclonal antibody (Catalog # A08379-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM2 at approximately 100KD. The expected band size for ADAM2 is at 82KD.
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