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Anti-ADAM2 Antibody

101-10025
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetADAM2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Mouse Anti-Human ADAM2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2515
  • Target name
    ADAM2
  • Target description
    ADAM metallopeptidase domain 2
  • Target synonyms
    cancer/testis antigen 15; CRYN1; CRYN2; CT15; disintegrin and metalloproteinase domain-containing protein 2; fertilin subunit beta; FTNB; PH30; PH-30b; PH30-beta
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Human ADAM2 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAM2 using anti-ADAM2 antibody (A08379-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat testis tissue lysate, lane 2: mouse testis tissue lysate, lane 3: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM2 antigen affinity purified polyclonal antibody (Catalog # A08379-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM2 at approximately 100KD. The expected band size for ADAM2 is at 82KD.
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