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Western blot All lanes: Disintegrin and metalloproteinase domain-containing protein 9 antibody at 2ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 91, 73 kDa Observed band size: 91 kDa
Western blot All lanes: Disintegrin and metalloproteinase domain-containing protein 9 antibody at 2ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 91, 73 kDa Observed band size: 91 kDa
Western blot All lanes: Disintegrin and metalloproteinase domain-containing protein 9 antibody at 2ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 91, 73 kDa Observed band size: 91 kDa

ADAM9 Antibody

CSB-PA618774ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetADAM9
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADAM9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8754
  • Target name
    ADAM9
  • Target description
    ADAM metallopeptidase domain 9
  • Target synonyms
    CORD9, MCMP, MDC9, Mltng, disintegrin and metalloproteinase domain-containing protein 9, ADAM metallopeptidase domain 9 (meltrin gamma), cellular disintegrin-related protein, cone rod dystrophy 9, metalloprotease/disintegrin/cysteine-rich protein 9, myeloma cell metalloproteinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13443
  • Protein Name
    Disintegrin and metalloproteinase domain-containing protein 9
  • Scientific Description
    Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ADAM9 using anti-ADAM9 antibody (A03074-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM9 antigen affinity purified polyclonal antibody (Catalog # A03074-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM9 at approximately 75 kDa. The expected band size for ADAM9 is at 91 kDa.
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